PGBD1 Polyklonaler Antikörper

PGBD1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 25539-1-AP

Synonyme

SCAND4, PiggyBac transposable element-derived protein 1, PGBD 1, hucep-4, HUCEP4

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  WB analysis of HeLa using 25539-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25539-1-AP (PGBD1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 25539-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 25539-1-AP (PGBD1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 25539-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 25539-1-AP (PGBD1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 25539-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25539-1-AP (PGBD1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 25539-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25539-1-AP (PGBD1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 25539-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25539-1-AP (PGBD1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse testis using 25539-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25539-1-AP (PGBD1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 25539-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25539-1-AP (PGBD1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 25539-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25539-1-AP (PGBD1 Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 25539-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PGBD1 antibody (25539-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, K-562-Zellen, Mauslebergewebe
• Erfolgreiche Detektion in IHC: Maushodengewebe, humanes Nierengewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

25539-1-AP bindet in WB, IHC, IF/ICC, ELISA PGBD1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PGBD1 fusion protein Ag19837
• Vollständiger Name: piggyBac transposable element derived 1
• Berechnetes Molekulargewicht: 809 aa, 93 kDa
• Beobachtetes Molekulargewicht: 85-90 kDa
• GenBank-Zugangsnummer: BC128585
• Gene symbol: PGBD1
• Gene ID (NCBI): 84547
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.