PGCP Rekombinanter Antikörper

PGCP Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

251059H3

Kat-Nr. : 86405-1-RR

Synonyme

Carboxypeptidase Q, CPQ, EC:3.4.17.-, LCH1, Lysosomal dipeptidase

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  WB analysis using 86405-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86405-1-RR (PGCP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. Negative control: mouse brain tissue

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  IF Staining of L02 using 86405-1-RR

  Immunofluorescent analysis of (4% PFA) fixed L02 cells using PGCP antibody (86405-1-RR, Clone: 251059H3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 86405-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86405-1-RR against Human PGCP were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattennierengewebe
• Erfolgreiche Detektion in IF/ICC: L02-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86405-1-RR bindet in WB, IF/ICC, ELISA PGCP und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PGCP fusion protein Ag9770
• Vollständiger Name: plasma glutamate carboxypeptidase
• Berechnetes Molekulargewicht: 472 aa, 52 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC020689
• Gene symbol: PGCP
• Gene ID (NCBI): 10404
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PGCP(plasma glutamate carboxypeptidase) is also named as LCH1,CPQ and belongs to the peptidase M28 family. It may play an important role in the hydrolysis of circulating peptides. It catalyzes more efficiently the hydrolysis of dipeptides with unsubstituted terminals into amino acids. It is a proteinase that acts on the unsubstituted N- and C-termini of dipeptides. It has been suggested that this PGCP is involved in the release of thyroxine(PMID:20951214). Human PGCP is a metallopeptidase with five potential glycosylation sites, whose activity depends on dimerizationand it can be detected a band of 65-72kd in FRTL-5 cells which is pro-PGCP(PMID:22127294). It can be N-glycosylated(PMID:10206990). It has the pro-form of 60-65 kDa and mature form of 55 kDa(PMID:20951214).