PGD Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PGD Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

17

Kat-Nr. : 14718-1-AP

Synonyme

6PGD, PGD, PGDH, phosphogluconate dehydrogenase

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Galerie der Validierungsdaten

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  WB analysis using 14718-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14718-1-AP (PGD antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 14718-1-AP

  IP Result of anti-PGD (IP:14718-1-AP, 4ug; Detection:14718-1-AP 1:500) with HepG2 cells lysate 3200ug.

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  IHC staining of human liver cancer using 14718-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14718-1-AP (PGD Antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 14718-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14718-1-AP (PGD Antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 14718-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 14718-1-AP (PGD Antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 14718-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 14718-1-AP (PGD Antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14718-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14718-1-AP (PGD Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 14718-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14718-1-AP (PGD Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lymphoma using 14718-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 14718-1-AP (PGD Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lymphoma using 14718-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 14718-1-AP (PGD Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 14718-1-AP

  Immunofluorescent analysis of HepG2 cells using 14718-1-AP (PGD antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:300-1:1200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 13 publications below
• IHC: See 4 publications below

Produktinformation

14718-1-AP bindet in WB, IP, IHC, IF, ELISA PGD und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PGD fusion protein Ag6444
• Vollständiger Name: phosphogluconate dehydrogenase
• Berechnetes Molekulargewicht: 53 kDa
• Beobachtetes Molekulargewicht: 53 kDa, 45 kDa
• GenBank-Zugangsnummer: BC000368
• Gene symbol: PGD
• Gene ID (NCBI): 5226
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Nat Commun; Polo-like kinase 1 coordinates biosynthesis during cell cycle progression by directly activating pentose phosphate pathway.; Authors - Xiaoyu Ma
mouse	WB	Clin Transl Med; SIRT5 deficiency enhances the proliferative and therapeutic capacities of adipose-derived mesenchymal stem cells via metabolic switching.; Authors - Tiantong Ou
human	IHC	J Invest Dermatol; Enhanced Glycogen Metabolism Supports the Survival and Proliferation of HPV-Infected Keratinocytes in Condylomata Acuminata.; Authors - Zhichao Gu
human	WB	Biochem Pharmacol; PMID: 32535103; Authors - Qi Feng
mouse	WB	iScience; The Symbiotic Relationship between the Neural Retina and Retinal Pigment Epithelium Is Supported by Utilizing Differential Metabolic Pathways.; Authors - Tirthankar Sinha
human	WB,IHC	Carcinogenesis; YTH domain family 2 promotes lung cancer cell growth by facilitating 6-phosphogluconate dehydrogenase mRNA translation.; Authors - Hao Sheng ; KD Validated