PGD Polyklonaler Antikörper

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• KD/KO Validated

PGD Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(25)

Kat-Nr. : 14718-1-AP

Synonyme

PGDH, EC:1.1.1.44, 6-phosphogluconate dehydrogenase, decarboxylating, 6PGD

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  WB analysis using 14718-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14718-1-AP (PGD antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 14718-1-AP

  IP result of anti-PGD (IP:14718-1-AP, 4ug; Detection:14718-1-AP 1:4000) with HepG2 cells lysate 1925 ug.

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  IHC staining of human liver cancer using 14718-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14718-1-AP (PGD Antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 14718-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14718-1-AP (PGD Antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 14718-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 14718-1-AP (PGD Antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 14718-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 14718-1-AP (PGD Antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14718-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14718-1-AP (PGD Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 14718-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14718-1-AP (PGD Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lymphoma using 14718-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 14718-1-AP (PGD Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lymphoma using 14718-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 14718-1-AP (PGD Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 14718-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PGD antibody (14718-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:300-1:1200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 20 publications below
• IHC: See 6 publications below

Produktinformation

14718-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA PGD und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PGD fusion protein Ag6444
• Vollständiger Name: phosphogluconate dehydrogenase
• Berechnetes Molekulargewicht: 53 kDa
• Beobachtetes Molekulargewicht: 53 kDa, 45 kDa
• GenBank-Zugangsnummer: BC000368
• Gene symbol: PGD
• Gene ID (NCBI): 5226
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PGD, also named as PGDH, belongs to the 6-phosphogluconate dehydrogenase family. PGD catalyses the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate in the context of the oxidative part of the pentose phosphate pathway. PGD is important for the production of NADPH, which is necessary for reductive biosynthesis, such as the formation of lipids and nucleotides, and the activity of enzymes involved in maintaining cell integrity, in combatting oxidative stress and in the first line of immunological defence. (PMID: 35234135)

Publikationen

Species	Application	Title
human	WB	Nat Commun; Polo-like kinase 1 coordinates biosynthesis during cell cycle progression by directly activating pentose phosphate pathway.; Authors - Xiaoyu Ma
mouse	WB	Clin Transl Med; SIRT5 deficiency enhances the proliferative and therapeutic capacities of adipose-derived mesenchymal stem cells via metabolic switching.; Authors - Tiantong Ou
human	IHC	J Invest Dermatol; Enhanced Glycogen Metabolism Supports the Survival and Proliferation of HPV-Infected Keratinocytes in Condylomata Acuminata.; Authors - Zhichao Gu
human	WB	Biochem Pharmacol; PMID: 32535103; Authors - Qi Feng
		J Biol Chem; Patient-derived organotypic tissue cultures as a platform to evaluate metabolic reprogramming in breast cancer patients; Authors - Teresa W -M Fan
mouse	WB	iScience; The Symbiotic Relationship between the Neural Retina and Retinal Pigment Epithelium Is Supported by Utilizing Differential Metabolic Pathways.; Authors - Tirthankar Sinha