PR Polyklonaler Antikörper

PR Polyklonal Antikörper für IF, IHC,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IHC, IF,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19980-1-AP

Synonyme

NR3C3, PGR, PR, progesterone receptor

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Galerie der Validierungsdaten

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  IHC staining of human breast cancer using 19980-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19980-1-AP (PR Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 19980-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19980-1-AP (PR Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 19980-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19980-1-AP (PR Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 19980-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19980-1-AP (PR Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 19980-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19980-1-AP (PR Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 19980-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19980-1-AP (PR Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 19980-1-AP

  Immunofluorescent analysis of MCF-7 cells using 19980-1-AP (PR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of MCF-7 using 19980-1-AP

  Immunofluorescent analysis of MCF-7 cells using 19980-1-AP (PR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

19980-1-AP bindet in IHC, IF,ELISA PR und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: progesterone receptor
• Berechnetes Molekulargewicht: 933 aa, 99 kDa
• GenBank-Zugangsnummer: NM_000926
• Gene symbol: PGR
• Gene ID (NCBI): 5241
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PGR, also named as NR3C3 and PR, belongs to the nuclear hormone receptor family and NR3 subfamily. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation.