PGT Rekombinanter Antikörper

PGT Rekombinant Antikörper für WB, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

230139F5

Kat-Nr. : 83302-1-PBS

Synonyme

SLCO2A1, PHOAR2, OATP2A1, 230139F5

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  WB analysis of mouse heart using 83302-1-RR (same clone as 83302-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 83302-1-RR (PGT antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83302-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 83302-1-RR (same clone as 83302-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 83302-1-RR (PGT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83302-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 83302-1-RR (same clone as 83302-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug PGT Recombinant antibody (83302-1-RR, Clone:230139F5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83302-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83302-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83302-1-RR against Human PGT were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

83302-1-PBS bindet in WB, FC (Intra), Indirect ELISA PGT und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PGT fusion protein Ag5594
• Vollständiger Name: solute carrier organic anion transporter family, member 2A1
• Berechnetes Molekulargewicht: 70 kDa
• Beobachtetes Molekulargewicht: 65 kDa
• GenBank-Zugangsnummer: BC041140
• Gene symbol: PGT
• Gene ID (NCBI): 6578
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Prostaglandin transporter (PGT), also known as SLCO2A1, has been identified as a functional prostaglandin (PG) carrier that mediates either the release of newly-synthesized PGs by cells and/or the uptake of PGs prior to intracellular oxidation. Northern blot analysis revealed that PGT mRNA has broad tissue distribution, suggesting its role in the transport and/ or metabolic clearance of PG in diverse tissues (PMID: 8787677,22331663).