Prohibitin Polyklonaler Antikörper

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• KD/KO Validated

Prohibitin Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

22

Rezensionen (1)

Kat-Nr. : 10787-1-AP

Synonyme

Cell and organelle markers, Mitochondria Marker, Mitochondrial Marker, Mitochondrion Marker, PHB, PHB1, prohibitin

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Galerie der Validierungsdaten

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  WB analysis using 10787-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10787-1-AP (Prohibitin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10787-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10787-1-AP (PHB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 10787-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10787-1-AP (PHB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 10787-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10787-1-AP (PHB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 10787-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 10787-1-AP (PHB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 10787-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10787-1-AP (PHB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 10787-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10787-1-AP (PHB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 10787-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10787-1-AP (Prohibitin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10787-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10787-1-AP (Prohibitin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 10787-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10787-1-AP (PHB antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 10787-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10787-1-AP (PHB antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 10787-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 10787-1-AP (Prohibitin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 10787-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Prohibitin antibody (10787-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of H9C2 using 10787-1-AP

  Immunofluorescent analysis of (4% PFA) fixed H9C2 cells using Prohibitin antibody (10787-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HeLa-Zellen, humanes Hirngewebe, humanes Lebergewebe, Jurkat-Zellen, Mausherzgewebe, Maushirngewebe, Mausnierengewebe, NIH/3T3-Zellen, Raji-Zellen, Rattenherzgewebe, Rattenhirngewebe, Rattennierengewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: H9C2-Zellen, HeLa-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 18 publications below
• IHC: See 3 publications below
• IF: See 3 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

10787-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA Prohibitin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Prohibitin fusion protein Ag1240
• Vollständiger Name: prohibitin
• Berechnetes Molekulargewicht: 30 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC013401
• Gene symbol: PHB
• Gene ID (NCBI): 5245
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Prohibitin, also known as PHB, a member of the Band-7 family of proteins, is widely distributed in bacteria, plants, yeast, protozoa and mammals and is important in cell proliferation, differentiation and apoptosis (PMID: 20840588 ). This protein localizes to the inner membrane of mitochondria, where it acts as a chaperone protein, and is also found in the nucleus, where it negatively regulates transcription (PMID: 18558096). Recent study confirmed that the expression and distribution of PHB, which is a nuclear matrix protein, affect the apoptosis of HaCaT cells and its co-localization with specific gene products connected with cell apoptosis (PMID: 24402549).

Publikationen

Species	Application	Title
human	WB	Cell; Cholesterol Transport through Lysosome-Peroxisome Membrane Contacts.; Authors - Bei-Bei Chu
human	WB	Autophagy; PHB2 (prohibitin 2) promotes PINK1-PRKN/Parkin-dependent mitophagy by the PARL-PGAM5-PINK1 axis.; Authors - Chaojun Yan
mouse	WB	Cell Death Differ; Mic60/Mitofilin determines MICOS assembly essential for mitochondrial dynamics and mtDNA nucleoid organization.; Authors - H Li
human	WB	Hum Mol Genet; Disease mutations in Rab7 result in unregulated nucleotide exchange and inappropriate activation.; Authors - McCray Brett A BA
mouse	WB	Cell Mol Gastroenterol Hepatol; DJ-1 deficiency in hepatocytes improves liver ischemia-reperfusion injury by enhancing mitophagy.; Authors - Min Xu
human	WB	Elife; Defining the interactome of the human mitochondrial ribosome identifies SMIM4 and TMEM223 as respiratory chain assembly factors.; Authors - Sven Dennerlein

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tom (Verified Customer) (02-24-2021)

HEK293T whole cell lysate. Membrane was blocked in 5% BSA for 1 hour then incubated overnight at 4 degrees with the primary antibody (1:1000)

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

Prohibitin Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:10787-1-AP)