PHD1 Polyklonaler Antikörper

PHD1 Polyklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12984-1-PBS

Synonyme

EGLN2, EC:1.14.11.-, EC:1.14.11.29, EIT6, EIT-6

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  WB analysis of mouse testis using 12984-1-AP (same clone as 12984-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12984-1-AP (PHD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12984-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 12984-1-AP (same clone as 12984-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12984-1-AP (PHD1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12984-1-PBS in a different storage buffer formulation.

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  WB analysis of human spleen using 12984-1-AP (same clone as 12984-1-PBS)

  human spleen tissue were subjected to SDS PAGE followed by western blot with 12984-1-AP (PHD1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12984-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 12984-1-AP (same clone as 12984-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12984-1-AP (PHD1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12984-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse heart using 12984-1-AP (same clone as 12984-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12984-1-AP (PHD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12984-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 12984-1-AP (same clone as 12984-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 12984-1-AP (PHD1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12984-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12984-1-PBS bindet in WB, Indirect ELISA PHD1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PHD1 fusion protein Ag3616
• Vollständiger Name: egl nine homolog 2 (C. elegans)
• Berechnetes Molekulargewicht: 407 aa, 44 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC036051
• Gene symbol: PHD1
• Gene ID (NCBI): 112398
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EGLN2, also named as PHD1, EIT6 and HPH-3, catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. It hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. EGLN2 functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. It may play a role in cell growth regulation.