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PHD1 Rekombinanter Antikörper

PHD1 Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

251511G2

Kat-Nr. : 86614-2-PBS

Synonyme

EGLN2, EC:1.14.11.-, EC:1.14.11.29, EIT6, EIT-6

Filter:
  • Western Blot (WB) analysis of various lysates using PHD1 Recombinant monoclonal antibody (86614-2-RR)

    WB analysis using 86614-2-RR (same clone as 86614-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86614-2-RR (EGLN2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86614-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PHD1 Recombinant monoclonal antibody (86614-2-RR)

    WB analysis using 86614-2-RR (same clone as 86614-2-PBS)

    Rat testis tissue were subjected to SDS PAGE followed by western blot with 86614-2-RR (EGLN2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86614-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86614-2-RR

    Affinity and Kinetic Characterization of 86614-2-RR

    Biolayer interferometry (BLl) kinetic assays of 86614-2-RR against Human PHD1 were performed. The affinity constant is 0.587 nM.

Geprüfte Anwendungen

Produktinformation

86614-2-PBS bindet in WB, Indirect ELISA PHD1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen PHD1 fusion protein Ag3616
Vollständiger Name egl nine homolog 2 (C. elegans)
Berechnetes Molekulargewicht 407 aa, 44 kDa
Beobachtetes Molekulargewicht48 kDa
GenBank-ZugangsnummerBC036051
Gene symbol PHD1
Gene ID (NCBI) 112398
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PHD1, also named as EGLN2, EIT6 and HPH-3, catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. It hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. EGLN2 functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. It may play a role in cell growth regulation.