PHD1 Rekombinanter Antikörper

PHD1 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

251511G2

Kat-Nr. : 86614-2-RR

Synonyme

EGLN2, EC:1.14.11.-, EC:1.14.11.29, EIT6, EIT-6

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  WB analysis using 86614-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86614-2-RR (EGLN2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 86614-2-RR

  Rat testis tissue were subjected to SDS PAGE followed by western blot with 86614-2-RR (EGLN2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 86614-2-RR

  Biolayer interferometry (BLl) kinetic assays of 86614-2-RR against Human PHD1 were performed. The affinity constant is 0.587 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, HEK-293-Zellen, HepG2-Zellen, Maushodengewebe, Rattenhodengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86614-2-RR bindet in WB, ELISA PHD1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PHD1 fusion protein Ag3616
• Vollständiger Name: egl nine homolog 2 (C. elegans)
• Berechnetes Molekulargewicht: 407 aa, 44 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC036051
• Gene symbol: PHD1
• Gene ID (NCBI): 112398
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PHD1, also named as EGLN2, EIT6 and HPH-3, catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. It hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. EGLN2 functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. It may play a role in cell growth regulation.