PHD2/EGLN1 Monoklonaler Antikörper

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• KD/KO Validated

PHD2/EGLN1 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1A2F1

Kat-Nr. : 66589-1-PBS

Synonyme

EGLN1, PHD2, EGLN 1, ECYT3, EC:1.14.11.29

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  WB analysis of mouse brain using 66589-1-Ig (same clone as 66589-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66589-1-Ig (EGLN1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66589-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66589-1-Ig (same clone as 66589-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66589-1-Ig (EGLN1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66589-1-PBS in a different storage buffer formulation.

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  WB analysis of SH-SY5Y using 66589-1-Ig (same clone as 66589-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 66589-1-Ig (EGLN1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66589-1-PBS in a different storage buffer formulation.

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  WB analysis of pig brain using 66589-1-Ig (same clone as 66589-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66589-1-Ig (EGLN1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66589-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 66589-1-Ig (same clone as 66589-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66589-1-Ig (EGLN1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66589-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 66589-1-Ig (same clone as 66589-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66589-1-Ig (EGLN1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66589-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66589-1-Ig (same clone as 66589-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66589-1-Ig (EGLN1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66589-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66589-1-Ig (same clone as 66589-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66589-1-Ig (EGLN1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66589-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 66589-1-Ig (same clone as 66589-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using PHD2/EGLN1 antibody (66589-1-Ig, Clone: 1A2F1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66589-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66589-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA PHD2/EGLN1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: egl nine homolog 1 (C. elegans)
• Berechnetes Molekulargewicht: 46 kDa
• Beobachtetes Molekulargewicht: 46 kDa, 44 kDa, 36 kDa
• GenBank-Zugangsnummer: NM_022051
• Gene symbol: PHD2/EGLN1
• Gene ID (NCBI): 54583
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EGLN1, also named as PHD2, SM-20, HPH-2 and HIF-PH2, catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. It hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. EGLN1 functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Defects in EGLN1 are the cause of erythrocytosis familial type 3 (ECYT3). EGLN1 has 3 isoforms with MW of 46 kDa, 44 kDa and 36 kDa produced by alternative splicing. It mainly localizes in cytoplasm and can shuttle between the nucleus and cytoplasm (PubMed:19631610). The antibody is specific to EGLN1.