PHEX Monoklonaler Antikörper

PHEX Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1D4B2

Kat-Nr. : 66934-1-PBS

Synonyme

1D4B2, EC:3.4.24.-, HPDR, HYP1, PEX

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  WB analysis of HEK-293 using 66934-1-Ig (same clone as 66934-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66934-1-Ig (PHEX antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66934-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat lung using 66934-1-Ig (same clone as 66934-1-PBS)

  rat lung tissue were subjected to SDS PAGE followed by western blot with 66934-1-Ig (PHEX antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66934-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig lung using 66934-1-Ig (same clone as 66934-1-PBS)

  pig lung tissue were subjected to SDS PAGE followed by western blot with 66934-1-Ig (PHEX antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66934-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66934-1-Ig (same clone as 66934-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66934-1-Ig (PHEX antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66934-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66934-1-PBS bindet in WB, IHC, Indirect ELISA PHEX und zeigt Reaktivität mit Hausschwein, human, Ratten

• Getestete Reaktivität: Hausschwein, human, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PHEX fusion protein Ag20290
• Vollständiger Name: phosphate regulating endopeptidase homolog, X-linked
• Berechnetes Molekulargewicht: 749 aa, 86 kDa
• Beobachtetes Molekulargewicht: 76-80 kDa, 95-100 kDa
• GenBank-Zugangsnummer: BC105059
• Gene symbol: PHEX
• Gene ID (NCBI): 5251
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PHEX encodes a transmembrane endopeptidase that belongs to the type II integral membrane zinc-dependent endopeptidase family. The protein usually expressed in osteocytes, osteoblasts and odontoblasts, and it was thought to be involved in bone and dentin mineralization and renal phosphate reabsorption (PMID:28982589). Otherwise, studies showed that PHEX transcripts were identified in human osteosarcoma-derived cells and in differentiated mouse osteoblasts, but not in immature mouse preosteoblasts, indicating the stage-specific expression of the protein (PMID:9199999). Defects in PHEX are responsible for X-linked hypophosphatemic rickets (HYP) (PMID:22339660). Glycosylation of proteins results in higher molecular weight, and about 95-100 kDa band were reported (PMID:25128529; PMID:18728349).