PHF14 Polyklonaler Antikörper

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• KD/KO Validated

PHF14 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 24787-1-PBS

Synonyme

KIAA0783, PHD finger protein 14

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  WB analysis of HeLa using 24787-1-AP (same clone as 24787-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 24787-1-AP (PHF14 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24787-1-PBS in a different storage buffer formulation.

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  WB analysis using 24787-1-AP (same clone as 24787-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 24787-1-AP (PHF14 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24787-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 24787-1-AP (same clone as 24787-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24787-1-AP (PHF14 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24787-1-PBS in a different storage buffer formulation.

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  WB analysis of MDA-MB-453s using 24787-1-AP (same clone as 24787-1-PBS)

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 24787-1-AP (PHF14 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24787-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 24787-1-AP (same clone as 24787-1-PBS)

  IP result of anti-PHF14 (IP:24787-1-AP, 4ug; Detection:24787-1-AP 1:500) with HEK-293 cells lysate 1200ug. This data was developed using the same antibody clone with 24787-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 24787-1-AP (same clone as 24787-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24787-1-AP (PHF14 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24787-1-PBS in a different storage buffer formulation.

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  IHC staining of human thyroid cancer using 24787-1-AP (same clone as 24787-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 24787-1-AP (PHF14 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24787-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 24787-1-AP (same clone as 24787-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24787-1-AP (PHF14 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24787-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

24787-1-PBS bindet in WB, IHC, IP, Indirect ELISA PHF14 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PHF14 fusion protein Ag20542
• Vollständiger Name: PHD finger protein 14
• Berechnetes Molekulargewicht: 888 aa, 100 kDa
• Beobachtetes Molekulargewicht: 140-150 kDa
• GenBank-Zugangsnummer: BC152414
• Gene symbol: PHF14
• Gene ID (NCBI): 9678
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PHF14 also named as KIAA0783 or PHD finger protein 14 is 888 amino acid protein, which contains 2 PHD-type znic fingers. PHF14 localizes in nucleus and may be involved in the regulation of transcription, DNA dependent. The function of PHF14 is still non-known.