PHF17 Polyklonaler Antikörper

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PHF17 Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 28472-1-PBS

Synonyme

Jade family PHD finger protein 1, JADE1, PHF 17, Protein Jade-1

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  WB analysis using 28472-1-AP (same clone as 28472-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28472-1-AP (PHF17 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28472-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 28472-1-AP (same clone as 28472-1-PBS)

  WB result of PHF17 antibody (28472-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PHF17 transfected HepG2 cells. This data was developed using the same antibody clone with 28472-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 28472-1-AP (same clone as 28472-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 28472-1-AP (PHF17 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28472-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 28472-1-AP (same clone as 28472-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 28472-1-AP (PHF17 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28472-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 28472-1-AP (same clone as 28472-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PHF17 antibody (28472-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28472-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

28472-1-PBS bindet in WB, IF/ICC, Indirect ELISA PHF17 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PHF17 fusion protein Ag29675
• Vollständiger Name: PHD finger protein 17
• Berechnetes Molekulargewicht: 96 kDa
• Beobachtetes Molekulargewicht: 65 kDa, 100-110 kDa
• GenBank-Zugangsnummer: BC032376
• Gene symbol: PHF17
• Gene ID (NCBI): 79960
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PHF17, also named as JADE1 and KIAA1807, belongs to the JADE family. PHF17 codes for the Jade-1 protein. This protein interacts with histone acetyltransferase HBO1 and promotes histone acetylation in chromatin to regulate DNA replication and gene transcription. PHF17 is mostly expressed in the small intestine, prostate, mammary gland and kidney. PHF17 is involved in mitosis, meiosis and tumor suppression (PMID: 28134766). PHF17 has 3 isoforms with the molecular mass of 58 and 94-96 kDa. With modification, the MW of PHF17 may be migrated to about 65 kDa and 100-110 kDa.