PHF19 Polyklonaler Antikörper

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• KD/KO Validated

PHF19 Polyklonal Antikörper für IF, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 11895-1-AP

Synonyme

hPCL3, MTF2L1, PCL3, PHD finger protein 19, PHF19, Polycomb like protein 3

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Galerie der Validierungsdaten

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  WB analysis of rat brain using 11895-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 11895-1-AP (PHF19 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of Mouse ES cells using 11895-1-AP

  IF result of Mouse ES cell From Kevin Corbit.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenhirngewebe
• Erfolgreiche Detektion in IF: Maus-ES-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 7 publications below
• IHC: See 2 publications below

Produktinformation

11895-1-AP bindet in WB, IHC, IF, ELISA PHF19 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PHF19 fusion protein Ag2511
• Vollständiger Name: PHD finger protein 19
• Berechnetes Molekulargewicht: 580 aa, 63 kDa
• Beobachtetes Molekulargewicht: 66 kDa
• GenBank-Zugangsnummer: BC022374
• Gene symbol: PHF19
• Gene ID (NCBI): 26147
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PHD FINGER PROTEIN 19(PHF19), a protein homologous to the Drosophila 'Polycomblike' (Pcl) protein, encoded by human gene PCL, which encodes 2 deduced proteins: a short form of 207 aa and a long form of 580 aa. The PCL family proteins contains a PCL homology domain, a Tudor domain, 2 PHD fingers and a C-terminal tail. It is highly expressed in thymus and heart, while 508aa form predominant in placenta, skeletal muscle and kidney and 207aa form in liver and peripheral blood leukocytes. Both of these forms can inhibit transcriptional activity. And the long form is corelated with cancer.Acts as a transcritpional repressor. The mRNA level of PHF19 is low in many normal tissue, it's elevated in cancer-derived cell lines.

Publikationen

Species	Application	Title
mouse	WB	Mol Cell; An H3K36 methylation-engaging Tudor motif of polycomb-like proteins mediates PRC2 complex targeting.; Authors - Ling Cai ; KD Validated
human		PLoS Genet; Alu Elements in ANRIL Non-Coding RNA at Chromosome 9p21 Modulate Atherogenic Cell Functions through Trans-Regulation of Gene Networks.; Authors - Holdt Lesca M LM
mouse	WB	PLoS Genet; Polycomb-like 3 promotes polycomb repressive complex 2 binding to CpG islands and embryonic stem cell self-renewal.; Authors - Julie Hunkapiller ; KD Validated
human	IHC	Front Immunol; Comprehensive Pan-Cancer Genomic Analysis Reveals PHF19 as a Carcinogenic Indicator Related to Immune Infiltration and Prognosis of Hepatocellular Carcinoma.; Authors - Zheng-Yi Zhu
human,mouse	WB	J Cancer; ZNF750 exerted its Antitumor Action in Oral Squamous Cell Carcinoma by regulating E2F2.; Authors - Hong-Li Yang
human	WB,IHC	Scand J Gastroenterol; Downregulation of PHF19 inhibits cell growth and migration in gastric cancer.; Authors - Haixiao Wang ; KD Validated