PHF19 Rekombinanter Antikörper

PHF19 Rekombinant Antikörper für WB, FC (Intra), Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241047D7

Kat-Nr. : 83892-3-PBS

Synonyme

241047D7, hPCL3, MTF2L1, PCL3, PHD finger protein 19

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  WB analysis using 83892-3-RR (same clone as 83892-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83892-3-RR (PHF19 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83892-3-PBS in a different storage buffer formulation.

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  FC experiment of A549 using 83892-3-RR (same clone as 83892-3-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.25 ug PHF19 Recombinant antibody (83892-3-RR, Clone:241047D7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83892-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00868-2

  Cytometric bead array standard curve of MP00868-2, PHF19 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83892-5-PBS. Detection antibody: 83892-3-PBS. Standard: Ag2511. Range: 0.313-40 ng/mL

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  Affinity and Kinetic Characterization of 83892-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83892-3-RR against Human PHF19 were performed. The affinity constant is 0.806 nM.

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Geprüfte Anwendungen

Produktinformation

83892-3-PBS bindet in WB, FC (Intra), Cytometric bead array, Indirect ELISA PHF19 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PHF19 fusion protein Ag2511
• Vollständiger Name: PHD finger protein 19
• Berechnetes Molekulargewicht: 580 aa, 63 kDa
• Beobachtetes Molekulargewicht: 62-66 kDa
• GenBank-Zugangsnummer: BC022374
• Gene symbol: PHF19
• Gene ID (NCBI): 26147
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PHD FINGER PROTEIN 19(PHF19), a protein homologous to the Drosophila 'Polycomblike' (Pcl) protein, encoded by human gene PCL, which encodes 2 deduced proteins: a short form of 207 aa and a long form of 580 aa. The PCL family proteins contains a PCL homology domain, a Tudor domain, 2 PHD fingers and a C-terminal tail. It is highly expressed in thymus and heart, while 508aa form predominant in placenta, skeletal muscle and kidney and 207aa form in liver and peripheral blood leukocytes. Both of these forms can inhibit transcriptional activity. And the long form is corelated with cancer.Acts as a transcritpional repressor. The mRNA level of PHF19 is low in many normal tissue, it's elevated in cancer-derived cell lines.