• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

PHF23 Polyklonaler Antikörper

PHF23 Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 25862-1-PBS

Synonyme

PDH-containing protein JUNE-1, hJUNE-1b, hJUNE 1b

Filter:
  • Western Blot (WB) analysis of various lysates using PHF23 Polyclonal antibody (25862-1-AP)

    WB analysis using 25862-1-AP (same clone as 25862-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25862-1-AP (PHF23 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25862-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using PHF23 Polyclonal antibody (25862-1-AP)

    WB analysis of HeLa using 25862-1-AP (same clone as 25862-1-PBS)

    WB result of PHF23 antibody (25862-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PHF23 transfected HeLa cells. This data was developed using the same antibody clone with 25862-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using PHF23 Polyclonal antibody (25862-1-AP)

    IF Staining of A549 using 25862-1-AP (same clone as 25862-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using PHF23 antibody (25862-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 25862-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

25862-1-PBS bindet in WB, IF/ICC, Indirect ELISA PHF23 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen PHF23 fusion protein Ag23044
Vollständiger Name PHD finger protein 23
Berechnetes Molekulargewicht 403 aa, 44 kDa
Beobachtetes Molekulargewicht~65 kDa
GenBank-ZugangsnummerBC002509
Gene symbol PHF23
Gene ID (NCBI) 79142
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PHD finger protein 23 (PHF23), also known as hJUNE-1b, belongs to the PHF23 family. As a putative epigenetic reader, the PHD domain of PHF23 specifically binds to tri-methylated lysine 4 of histone H3 (H3K4me3). PHF23 is widely expressed in human tissues and various cell lines and exerts a negative effect on autophagy by promoting the degradation of E3 ligase LRSAM1 (leucine-rich repeat and sterile alpha motif containing 1) (PMID: 32978226, 32380078). The observed molecular weight of PHF23 is ~65 kDa, which may be caused by post-translational modification. The specificity of this antibody was demonstrated by siRNA-mediated knockdown of target protein.