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PHF5A Monoklonaler Antikörper

PHF5A Monoklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1G7E2

Kat-Nr. : 68196-1-PBS

Synonyme

bK223H9.2, INI, PHD finger protein 5A, PHF5A, SF3b14b

Filter:
  • Western Blot (WB) analysis of various lysates using PHF5A Monoclonal antibody (68196-1-Ig)

    WB analysis using 68196-1-Ig (same clone as 68196-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68196-1-Ig (PHF5A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68196-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using PHF5A Monoclonal antibody (68196-1-Ig)

    WB analysis of NIH/3T3 using 68196-1-Ig (same clone as 68196-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68196-1-Ig (PHF5A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68196-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

68196-1-PBS bindet in WB, Indirect ELISA PHF5A und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen PHF5A fusion protein Ag7804
Vollständiger Name PHD finger protein 5A
Berechnetes Molekulargewicht 12 kDa
Beobachtetes Molekulargewicht12 kDa
GenBank-ZugangsnummerBC007321
Gene symbol PHF5A
Gene ID (NCBI) 84844
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The PHD finger-like domain protein 5a (PHF5A) is ubiquitously expressed and is located in the nucleus. The gene Phf5a and its human counterpart PHF5A (former name Ini) are located on rat chromosome 7 (RNO7q34) and human chromosome 22 (HSA2213q2), respectively. The rat gene consists of four exons, while the human gene have five exons. Both genes encode a highly conserved protein of 110 amino acids that contains a PHD finger domain.(PMID: 23675859) It acts as a transcriptional regulator by binding to the GJA1/Cx43 promoter and enhancing its up-regulation by ESR1/ER-alpha and is involved in pre-mRNA splicing.