PHF8 Polyklonaler Antikörper

PHF8 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29516-1-PBS

Synonyme

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  WB analysis using 29516-1-AP (same clone as 29516-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 29516-1-AP (PHF8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29516-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 29516-1-AP (same clone as 29516-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29516-1-AP (PHF8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29516-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 29516-1-AP (same clone as 29516-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29516-1-AP (PHF8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29516-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

29516-1-PBS bindet in WB, IHC, Indirect ELISA PHF8 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PHF8 fusion protein Ag30701
• Vollständiger Name: PHD finger protein 8
• Berechnetes Molekulargewicht: 118 kDa
• Beobachtetes Molekulargewicht: 125-135 kDa
• GenBank-Zugangsnummer: NM_001184896
• Gene symbol: PHF8
• Gene ID (NCBI): 23133
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Plant homeodomain finger protein 8 (PHF8) is a JmjC domain-containing protein. PHF8 is localized in the nucleolus and may regulate rRNA transcription. As a member of the KDM7 subfamily of enzymes, PHF8 is involved in demethylation of lysine residues in histones such as H3K27me2/1, H3K9me2/1 and H4K20me1. PHF8 has been reported to participate in cancer development and metastasis of various types of tumors(PMID: 33111613). PHF8 has 5 isoforms produced by alternative splicing(Uniprot).