PHIP Polyklonaler Antikörper

PHIP Polyklonal Antikörper für WB, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20933-1-PBS

Synonyme

---

• 

  WB analysis using 20933-1-AP (same clone as 20933-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20933-1-AP (PHIP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20933-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A375 using 20933-1-AP (same clone as 20933-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 20933-1-AP (PHIP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20933-1-PBS in a different storage buffer formulation.

• 

  IP experiment of A375 using 20933-1-AP (same clone as 20933-1-PBS)

  IP result of anti-PHIP (IP:20933-1-AP, 4ug; Detection:20933-1-AP 1:3000) with A375 cells lysate 3600ug. This data was developed using the same antibody clone with 20933-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 20933-1-AP (same clone as 20933-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 20933-1-AP (PHIP antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20933-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

20933-1-PBS bindet in WB, IF/ICC, IP, Indirect ELISA PHIP und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PHIP fusion protein Ag14968
• Vollständiger Name: pleckstrin homology domain interacting protein
• Berechnetes Molekulargewicht: 1821 aa, 207 kDa
• Beobachtetes Molekulargewicht: 207 kDa
• GenBank-Zugangsnummer: BC008909
• Gene symbol: PHIP
• Gene ID (NCBI): 55023
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PHIP is a protein that binds to the insulin receptor substrate 1 protein and regulates glucose transporter translocation in skeletal muscle cells. PHIP may also regulate growth and survival of pancreatic beta cells. Elevated copy number of this gene may be associated with melanoma severity and the encoded protein may promote melanoma metastasis in human patients.