PHLDA1 Rekombinanter Antikörper

PHLDA1 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

243156B7

Kat-Nr. : 85783-1-RR

Synonyme

Apoptosis-associated nuclear protein, DT1P1B11, PHRIP, Pleckstrin homology-like domain family A member 1, PQ-rich protein

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  WB analysis using 85783-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85783-1-RR (PHLDA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 85783-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85783-1-RR (PHLDA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 85783-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85783-1-RR against Human PHLDA1 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MDA-MB-231-Zellen, A375-Zellen, BxPC-3-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85783-1-RR bindet in WB, ELISA PHLDA1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PHLDA1 fusion protein Ag13125
• Vollständiger Name: pleckstrin homology-like domain, family A, member 1
• Berechnetes Molekulargewicht: 45 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC018929
• Gene symbol: PHLDA1
• Gene ID (NCBI): 22822
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PHLDA1, also known as PHRIP and TDAG51, is a multifunctional protein involved in various biological processes. It can induce apoptosis in various cell types, including T cells, hippocampal cells, endothelial cells, melanoma cells, and mouse embryonic fibroblasts(PMID: 30207029). PHLDA1 plays a role in inhibiting growth factor signaling and has been implicated in tumor suppression through its ability to repress Akt activity by binding to phosphatidylinositol (PIP) lipids(PMID: 36142223).