PHLDB2 Polyklonaler Antikörper

PHLDB2 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27940-1-PBS

Synonyme

Protein LL5-beta, Pleckstrin homology-like domain family B member 2, LL5beta, LL5B

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  WB analysis of A431 using 27940-1-AP (same clone as 27940-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 27940-1-AP (PHLDB2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27940-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 27940-1-AP (same clone as 27940-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27940-1-AP (PHLDB2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27940-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 27940-1-AP (same clone as 27940-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27940-1-AP (PHLDB2 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27940-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 27940-1-AP (same clone as 27940-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27940-1-AP (PHLDB2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27940-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 27940-1-AP (same clone as 27940-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27940-1-AP (PHLDB2 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27940-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 27940-1-AP (same clone as 27940-1-PBS)

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 27940-1-AP (PHLDB2 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27940-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 27940-1-AP (same clone as 27940-1-PBS)

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 27940-1-AP (PHLDB2 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27940-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin cancer using 27940-1-AP (same clone as 27940-1-PBS)

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 27940-1-AP (PHLDB2 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27940-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

27940-1-PBS bindet in WB, IHC, Indirect ELISA PHLDB2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PHLDB2 fusion protein Ag27331
• Vollständiger Name: pleckstrin homology-like domain, family B, member 2
• Berechnetes Molekulargewicht: 1253 aa, 142 kDa
• Beobachtetes Molekulargewicht: 150 kDa
• GenBank-Zugangsnummer: BC142678
• Gene symbol: PHLDB2
• Gene ID (NCBI): 90102
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The pleckstrin homology-like domain (PHLD) class of proteins are multifunctional proteins comprised of two families of proteins, PHLDA and PHLDB, each with 3 members: PHLDA1, PHLDA2, PHDLA3, PHLDB1, PHLDB2, and PHLDB3 (PMID: 32429563). All the family members possess a pleckstrin homology (PH) domain. PHLB2, also known as LL5-beta, contains two predicted coiled-coil domains, a serine-rich region predicted to contain multiple protein kinase C phosphorylation sites in addition to a PH domain (PMID: 15851520). PHLB2 is a filamin-binding protein that is an effector of the PI3K signaling pathway (PMID: 17174070). It is a PtdIns(3,4,5)P3 (PIP3) binding protein and can translocate to the plasma membrane in a receptor and PI3K-dependant manner (PMID: 12376540). PHLB2 has also been reported as a component of the postsynaptic apparatus and a regulator of postsynaptic differentiation (PMID: 15851520).