PHLPP Monoklonaler Antikörper

PHLPP Monoklonal Antikörper für WB,ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

3

CloneNo.

2E8E6

Kat-Nr. : 67640-1-Ig

Synonyme

hSCOP, KIAA0606, PHLPP, PHLPP1, PLEKHE1, SCOP

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Galerie der Validierungsdaten

• 

  WB analysis of mouse brain using 67640-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67640-1-Ig (PHLPP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 67640-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67640-1-Ig (PHLPP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 67640-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67640-1-Ig (PHLPP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 67640-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67640-1-Ig (PHLPP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Hausschwein-Hirngewebe, HEK-293-Zellen, Rattenhirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

67640-1-Ig bindet in WB, ELISA PHLPP und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PHLPP fusion protein Ag18982
• Vollständiger Name: PH domain and leucine rich repeat protein phosphatase
• Berechnetes Molekulargewicht: 1717 aa, 184 kDa
• Beobachtetes Molekulargewicht: 185 kDa
• GenBank-Zugangsnummer: BC126277
• Gene symbol: PHLPP
• Gene ID (NCBI): 23239
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PHLPP (PH domain leucine-rich-repeats protein phosphatase) belongs to a novel family of Ser/Thr protein phosphatases that consists of PHLPP1 and PHLPP2 isoforms. It has been shown that PHLPP negatively regulates multiple oncogenic pathways by directly dephosphorylating and inactivating key signaling molecules, including Akt, S6K, and RAF1. Observed MW of PHLPP is 185 kDa.

Publikationen

Species	Application	Title
mouse	WB	Oxid Med Cell Longev; MicroRNA-299a-5p Protects against Spinal Cord Injury through Activating AMPK Pathway.; Authors - Zong-Ze Zhang
pig	WB	Pediatr Res; Preterm birth alters the feeding-induced activation of Akt signaling in the muscle of neonatal piglets; Authors - Agus Suryawan
human	WB	Cell Death Dis; Direct conversion of human umbilical cord mesenchymal stem cells into retinal pigment epithelial cells for treatment of retinal degeneration; Authors - Xiaoman Zhu