PHLPP2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PHLPP2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 25244-1-PBS

Synonyme

PHLPPL, EC:3.1.3.16, KIAA0931, PH domain leucine-rich repeat-containing protein phosphatase 2, PH domain leucine-rich repeat-containing protein phosphatase-like

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  WB analysis using 25244-1-AP (same clone as 25244-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 25244-1-AP (PHLPP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25244-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 25244-1-AP (same clone as 25244-1-PBS)

  WB result of PHLPP2 antibody (25244-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PHLPP2 transfected HeLa cells. This data was developed using the same antibody clone with 25244-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 25244-1-AP (same clone as 25244-1-PBS)

  IP result of anti-PHLPP2 (IP:25244-1-AP, 4ug; Detection:25244-1-AP 1:500) with HeLa cells lysate 1880 ug. This data was developed using the same antibody clone with 25244-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 25244-1-AP (same clone as 25244-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25244-1-AP (PHLPP2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 25244-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 25244-1-AP (same clone as 25244-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25244-1-AP (PHLPP2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 25244-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 25244-1-AP (same clone as 25244-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25244-1-AP (PHLPP2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 25244-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 25244-1-AP (same clone as 25244-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25244-1-AP (PHLPP2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 25244-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 25244-1-AP (same clone as 25244-1-PBS)

  Immunofluorescent analysis of HeLa cells using 25244-1-AP (PHLPP2 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 25244-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

25244-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA PHLPP2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PHLPP2 fusion protein Ag19000
• Vollständiger Name: PH domain and leucine rich repeat protein phosphatase-like
• Berechnetes Molekulargewicht: 1323 aa, 147 kDa
• Beobachtetes Molekulargewicht: 147 kDa
• GenBank-Zugangsnummer: BC129927
• Gene symbol: PHLPPL
• Gene ID (NCBI): 23035
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PHLPP2 also named as PHLPPL is a 1323 amino acid protein, which contains 22 LRR repeats, 1 PH domain and 1 PP2C-like domain. Localized to both the nucleus and the cytoplasm, PHLPPL uses manganese as a cofactor and mediates the dephosphorylation of Akt1, thereby playing a role in cell survival and apoptotic regulation. Multiple isoforms of PHLPPL exist due to alternative splicing events.