PHOX2B Monoklonaler Antikörper

PHOX2B Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

CloneNo.

1F5F5

Kat-Nr. : 66254-1-Ig

Synonyme

NBPhox, Neuroblastoma Phox, paired like homeobox 2b, PHOX2B, PHOX2B homeodomain protein, PMX2B

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  WB analysis of Neuro-2a using 66254-1-Ig

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66254-1-Ig (PHOX2B Antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IHC staining of neuroblastoma using 66254-1-Ig

  Immunohistochemical analysis of paraffin-embedded neuroblastoma tissue slide using 66254-1-Ig (PHOX2B antibody) at dilution of 1:300 (under 40x lens).

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  IHC staining of neuroblastoma using 66254-1-Ig

  Immunohistochemical analysis of paraffin-embedded neuroblastoma tissue slide using 66254-1-Ig (PHOX2B antibody) at dilution of 1:300 (under 10x lens).

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  IF Staining of Neuro-2a using 66254-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using PHOX2B antibody (66254-1-Ig, Clone: 1F5F5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

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  IF Staining of SH-SY5Y using 66254-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using PHOX2B antibody (66254-1-Ig, Clone: 1F5F5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Neuro-2a-Zellen
• Erfolgreiche Detektion in IHC: Neuroblastomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: SH-SY5Y-Zellen, Neuro-2a-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 3 publications below

Produktinformation

66254-1-Ig bindet in WB, IHC, IF/ICC, ELISA PHOX2B und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PHOX2B fusion protein Ag13196
• Vollständiger Name: paired-like homeobox 2b
• Berechnetes Molekulargewicht: 31 kDa
• Beobachtetes Molekulargewicht: 32-36 kDa
• GenBank-Zugangsnummer: BC017199
• Gene symbol: PHOX2B
• Gene ID (NCBI): 8929
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Paired mesoderm homeobox protein 2B (PHOX2B), also named as Neuroblastoma Phox, is a 314 amino acid protein, which contains one homeobox DNA-binding domain and belongs to the paired homeobox family. PHOX2B localizes in the nucleus and is expressed in neuroblastoma, brain and adrenal gland. PHOX2B plays a role in the development of several major noradrenergic neuron populations, including the locus coeruleus. PHOX2B has an association with congenital central hypoventilation syndrome.

Publikationen

Species	Application	Title
human	IF	Sci Signal; Mitotic cell responses to substrate topological cues are independent of the molecular nature of adhesion.; Authors - Ouranio Anastasiou
mouse	IF	Nat Biotechnol; Generation of locus coeruleus norepinephrine neurons from human pluripotent stem cells; Authors - Yunlong Tao
mouse	IF	Dev Growth Differ; Chromosomal localization of PHOX2B during M-phase is disrupted in disease-associated mutants; Authors - Yuki Sato