PHYH Polyklonaler Antikörper

PHYH Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 12858-1-AP

Synonyme

LN1, LNAP1, PAHX, PHYH, PHYH1, Phytanic acid oxidase, phytanoyl CoA 2 hydroxylase, RD

---

• 

  WB analysis of human liver using 12858-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12858-1-AP (PHYH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 12858-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12858-1-AP (PHYH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12858-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12858-1-AP (PHYH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 12858-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12858-1-AP (PHYH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 12858-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 12858-1-AP (PHYH antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 12858-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 12858-1-AP (PHYH antibody) at dilution of 1:50 (under 40x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Lebergewebe, HEK-293-Zellen, humanes Nierengewebe, Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IHC: See 1 publications below

Produktinformation

12858-1-AP bindet in WB, IHC, ELISA PHYH und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PHYH fusion protein Ag3569
• Vollständiger Name: phytanoyl-CoA 2-hydroxylase
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 36 kDa, 70 kDa
• GenBank-Zugangsnummer: BC029512
• Gene symbol: PHYH
• Gene ID (NCBI): 5264
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PHYH (Phytanoyl-CoA 2-hydroxylase), also known as Phytanic acid oxidase or Phytanoyl-CoA alpha-hydroxylase, is a peroxisomal enzyme which converts phytanoyl-CoA to 2-hydroxyphytanoyl-CoA. It catalyzes the first step in the alpha-oxidation of phytanic acid, a branched-chain fatty acid. Mutations in this gene can cause Refsum disease (RD) and deficient protein activity can cause Zellweger syndrome and rhizomelic chondrodysplasia punctata. This antibody (12858-1-AP) detects two bands: 36kd and 70kd. The 70kd band may probably be the dimeric form of PHYH.

Publikationen

Species	Application	Title
mouse	WB	PLoS Genet; Tysnd1 deficiency in mice interferes with the peroxisomal localization of PTS2 enzymes, causing lipid metabolic abnormalities and male infertility.; Authors - Mizuno Yumi Y
rat	WB	Oncotarget; Proteomics and metabolomics analysis of hepatic mitochondrial metabolism in alcohol-preferring and non-preferring rats.; Authors - Hao-Long Zeng
pig	WB	Sci Rep; Proteomics analysis reveals the effect of 1α,25(OH)2VD3-glycosides on development of early testes in piglets.; Authors - Haodong Chen
rat	WB	J Ethnopharmacol; Isobaric tags for relative and absolute quantitation (iTRAQ)-based proteomics for the investigation of the effect of Hugan Qingzhi on non-alcoholic fatty liver disease in rats.; Authors - Xiaorui Yao
mouse	WB,IHC	J Hepatocell Carcinoma; Liver Regeneration-Related Genes of Nontumor Liver Tissues Predict the Prognosis of Patients with Hepatocellular Carcinoma; Authors - Yanchao Xu
human	WB	Nat Cell Biol; PEX39 facilitates the peroxisomal import of PTS2-containing proteins; Authors - Walter W Chen