PHYHIPL Polyklonaler Antikörper

PHYHIPL Polyklonal Antikörper für WB, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF-P, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16937-1-PBS

Synonyme

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  WB analysis using 16937-1-AP (same clone as 16937-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16937-1-AP (PHYHIPL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16937-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 16937-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16937-1-AP (PHYHIPL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 16937-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16937-1-AP (PHYHIPL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 16937-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16937-1-AP (PHYHIPL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 16937-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16937-1-AP (PHYHIPL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 16937-1-AP (same clone as 16937-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 16937-1-AP (PHYHIPL antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16937-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

16937-1-PBS bindet in WB, IF-P, ELISA PHYHIPL und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PHYHIPL fusion protein Ag10435
• Vollständiger Name: phytanoyl-CoA 2-hydroxylase interacting protein-like
• Berechnetes Molekulargewicht: 376 aa, 43 kDa
• Beobachtetes Molekulargewicht: 39 kDa and 42-45 kDa
• GenBank-Zugangsnummer: BC011268
• Gene symbol: PHYHIPL
• Gene ID (NCBI): 84457
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PHYHIPL, a kind of mitochondrial protein, is associated with energy production, oxidative stress and organelle dynamics. It is reported that global ischemia is related to the change of PHYHIPL protein level. Compared with the control group, the level of APOE (may be involved in abnormal regulation of mitochondrial function) is significantly increased after ischemic stimulation, while the APOE genotype has a effect on PHYHIPL protein level. 16937-1-AP detects 39 kDa isoform and 42-45 kDa isoform in SDS-PAGE. (PMID: 21878944)