PI16 Polyklonaler Antikörper

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• KD/KO Validated

PI16 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 12267-1-AP

Synonyme

CD364, CRISP 9, CRISP9, CRISP-9, Cysteine-rich secretory protein 9

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  WB analysis using 12267-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12267-1-AP (PI16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12267-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12267-1-AP (PI16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A2780 using 12267-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 12267-1-AP (PI16 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 12267-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12267-1-AP (PI16 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 12267-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12267-1-AP (PI16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 12267-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12267-1-AP (PI16 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 12267-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12267-1-AP (PI16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 12267-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12267-1-AP (PI16 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 12267-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12267-1-AP (PI16 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 12267-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12267-1-AP (PI16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SKOV-3-Zellen, A2780-Zellen, A549-Zellen, COLO 320-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Pankreaskarzinomgewebe, Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

12267-1-AP bindet in WB, IHC, IF, CoIP, ELISA PI16 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PI16 fusion protein Ag2925
• Vollständiger Name: peptidase inhibitor 16
• Berechnetes Molekulargewicht: 463 aa, 49 kDa
• Beobachtetes Molekulargewicht: 49 kDa
• GenBank-Zugangsnummer: BC022399
• Gene symbol: PI16
• Gene ID (NCBI): 221476
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Peptidase inhibitor 16 (PI16) is localized to chromosome 6p21.2 in human, which is also called as prostate secretory protein 94-binding protein (PSPBP). It is a member of the cysteine-rich secretory proteins, antigen 5, and pathogenesis-related proteins (CAP) superfamily (PMID: 32779397). PI16 is a cysteine-rich protein that is widely expressed in human tissuest (PMID: 18824526). PI16 is involved in extracellular matrix regulation, acts as a tumour suppressor gene and is a new independent prognostic marker of prostate cancer (PMID: 37525118, PMID: 17062675).

Publikationen

Species	Application	Title
mouse,human	WB,IHC,CoIP	Cell Mol Biol Lett; Peptidase inhibitor (PI16) impairs bladder cancer metastasis by inhibiting NF-κB activation via disrupting multiple-site ubiquitination of NEMO; Authors - Xiangqin Kuang ; KD Validated
human	IF	Clin Cancer Res; Single-cell transcriptomic landscape deciphers intratumoral heterogeneity and subtypes of acral and mucosal melanoma; Authors - Yunyan Li