PI16 Polyklonaler Antikörper

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• KD/KO Validated

PI16 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12267-1-PBS

Synonyme

CD364, CRISP 9, CRISP9, CRISP-9, Cysteine-rich secretory protein 9

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  WB analysis using 12267-1-AP (same clone as 12267-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12267-1-AP (PI16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12267-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 12267-1-AP (same clone as 12267-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12267-1-AP (PI16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12267-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 12267-1-AP (same clone as 12267-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12267-1-AP (PI16 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12267-1-PBS in a different storage buffer formulation.

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  WB analysis of A2780 using 12267-1-AP (same clone as 12267-1-PBS)

  A2780 cells were subjected to SDS PAGE followed by western blot with 12267-1-AP (PI16 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12267-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 12267-1-AP (same clone as 12267-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12267-1-AP (PI16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12267-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 12267-1-AP (same clone as 12267-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12267-1-AP (PI16 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12267-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 12267-1-AP (same clone as 12267-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12267-1-AP (PI16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12267-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 12267-1-AP (same clone as 12267-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12267-1-AP (PI16 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12267-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 12267-1-AP (same clone as 12267-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12267-1-AP (PI16 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12267-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 12267-1-AP (same clone as 12267-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12267-1-AP (PI16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12267-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12267-1-PBS bindet in WB, IHC, ELISA PI16 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PI16 fusion protein Ag2925
• Vollständiger Name: peptidase inhibitor 16
• Berechnetes Molekulargewicht: 463 aa, 49 kDa
• Beobachtetes Molekulargewicht: 49 kDa
• GenBank-Zugangsnummer: BC022399
• Gene symbol: PI16
• Gene ID (NCBI): 221476
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Peptidase inhibitor 16 (PI16) is localized to chromosome 6p21.2 in human, which is also called as prostate secretory protein 94-binding protein (PSPBP). It is a member of the cysteine-rich secretory proteins, antigen 5, and pathogenesis-related proteins (CAP) superfamily (PMID: 32779397). PI16 is a cysteine-rich protein that is widely expressed in human tissuest (PMID: 18824526). PI16 is involved in extracellular matrix regulation, acts as a tumour suppressor gene and is a new independent prognostic marker of prostate cancer (PMID: 37525118, PMID: 17062675).