PI16 Polyklonaler Antikörper

PI16 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30527-1-AP

Synonyme

CD364, CRISP 9, CRISP9, CRISP-9, Cysteine-rich secretory protein 9

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  WB analysis using 30527-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30527-1-AP (PI16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 30527-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30527-1-AP (PI16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of SKOV-3 using 30527-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using PI16 antibody (30527-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, Rattenherzgewebe, SKOV-3-Zellen
• Erfolgreiche Detektion in IF/ICC: SKOV-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

30527-1-AP bindet in WB, IF/ICC, ELISA PI16 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PI16 fusion protein Ag32556
• Vollständiger Name: peptidase inhibitor 16
• Berechnetes Molekulargewicht: 463 aa, 49 kDa
• Beobachtetes Molekulargewicht: 100 kDa, 49 kDa
• GenBank-Zugangsnummer: BC022399
• Gene symbol: PI16
• Gene ID (NCBI): 221476
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Peptidase inhibitor 16 (PI16) is localized to chromosome 6p21.2 in human, which is also called as prostate secretory protein 94-binding protein (PSPBP). It is a member of the cysteine-rich secretory proteins, antigen 5, and pathogenesis-related proteins (CAP) superfamily (PMID: 32779397). PI16 is a cysteine-rich protein that is widely expressed in human tissuest (PMID: 18824526). PI16 is involved in extracellular matrix regulation, acts as a tumour suppressor gene and is a new independent prognostic marker of prostate cancer (PMID: 37525118, PMID: 17062675).