PI3 Kinase p85 Beta Monoklonaler Antikörper

PI3 Kinase p85 Beta Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2B3B6

Kat-Nr. : 67644-1-PBS

Synonyme

p85, p85 BETA, P85B, PI3 Kinase p85 Beta, PI3 kinase subunit p85 beta, PI3K regulatory subunit beta, PIK3R2

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  WB analysis using 67644-1-Ig (same clone as 67644-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67644-1-Ig (PI3 Kinase p85 Beta antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67644-1-PBS in a different storage buffer formulation.

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  WB analysis using 67644-1-Ig (same clone as 67644-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67644-1-Ig (PIK3R2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67644-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 67644-1-Ig (same clone as 67644-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67644-1-Ig (PIK3R2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67644-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 67644-1-Ig (same clone as 67644-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67644-1-Ig (PIK3R2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67644-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67644-1-Ig (same clone as 67644-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PI3 Kinase p85 Beta antibody (67644-1-Ig, Clone: 2B3B6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 67644-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67644-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA PI3 Kinase p85 Beta und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PI3 Kinase p85 Beta fusion protein Ag14369
• Vollständiger Name: phosphoinositide-3-kinase, regulatory subunit 2 (beta)
• Berechnetes Molekulargewicht: 728 aa, 82 kDa
• Beobachtetes Molekulargewicht: 85 kDa
• GenBank-Zugangsnummer: BC014170
• Gene symbol: PI3 Kinase p85 Beta
• Gene ID (NCBI): 5296
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PI3 Kinase p85 is also named as PIK3R2 and belongs to the PI3K p85 subunit family. PI3 Kinase p85 is a regulatory subunit of phosphoinositide-3-kinase (PI3K) which is a kinase that phosphorylates phosphatidylinositol 4,5-bisphosphate to generate PIP3. PI3 Kinase p85 binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain, and then acts as an adapter to mediate the association of the p110 catalytic unit to the plasma membrane. It promotes nuclear translocation of XBP1 isoform 2 in a ER stress or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PMID:23604317).