PI3 Kinase p85 Beta Polyklonaler Antikörper

PI3 Kinase p85 Beta Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 25868-1-AP

Synonyme

p85, p85 BETA, P85B, PI3 Kinase p85 Beta, PI3 kinase subunit p85 beta, PI3K p85(beta), PI3K regulatory subunit beta, PIK3R2

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  WB analysis using 25868-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 25868-1-AP (PI3 Kinase p85 Beta antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 25868-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 25868-1-AP (PI3 Kinase p85 Beta antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 25868-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25868-1-AP (PI3 Kinase p85 Beta antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 25868-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 25868-1-AP (PI3 Kinase p85 Beta antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse small intestine using 25868-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 25868-1-AP (PI3 Kinase p85 Beta antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 25868-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 25868-1-AP (PI3 Kinase p85 Beta antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 25868-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 25868-1-AP (PI3 Kinase p85 Beta antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat small intestine using 25868-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 25868-1-AP (PI3 Kinase p85 Beta antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat small intestine using 25868-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 25868-1-AP (PI3 Kinase p85 Beta antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, U-937-Zellen
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe, Mausnierengewebe, Maus-Dünndarmgewebe, Rattennierengewebe, Ratten-Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

25868-1-AP bindet in WB, IHC, ELISA PI3 Kinase p85 Beta und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PI3 Kinase p85 Beta fusion protein Ag14369
• Vollständiger Name: phosphoinositide-3-kinase, regulatory subunit 2 (beta)
• Berechnetes Molekulargewicht: 728 aa, 82 kDa
• Beobachtetes Molekulargewicht: 82 kDa
• GenBank-Zugangsnummer: BC014170
• Gene symbol: PI3 Kinase p85 Beta
• Gene ID (NCBI): 5296
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PI3 Kinase p85 is also named as PIK3R2 and belongs to the PI3K p85 subunit family. PI3 Kinase p85 is a regulatory subunit of phosphoinositide-3-kinase (PI3K) which is a kinase that phosphorylates phosphatidylinositol 4,5-bisphosphate to generate PIP3. PI3 Kinase p85 binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain, and then acts as an adapter to mediate the association of the p110 catalytic unit to the plasma membrane. It promotes nuclear translocation of XBP1 isoform 2 in a ER stress or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PMID:23604317).

Publikationen

Species	Application	Title
mouse	WB	Front Pharmacol; Aconiti Lateralis Radix Praeparata ameliorates heart failure via PI3K/AKT/Bnip3 pathway; Authors - Wenxiu Liu
rat	WB	Nanomaterials (Basel); 2D MXene Nanosheets with ROS Scavenging Ability Effectively Delay Osteoarthritis Progression; Authors - Hongqi Zhao
human	WB	Int J Mol Sci; miR-212-5p Regulates PM2.5-Induced Apoptosis by Targeting LAMC2 and LAMA3; Authors - Yunna Jia