PI4KA Rekombinanter Antikörper

PI4KA Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

230057G2

Kat-Nr. : 82938-1-PBS

Synonyme

PI4-kinase alpha, PI4K-alpha, Phosphatidylinositol 4-Kinase III alpha, Phosphatidylinositol 4-kinase alpha, EC:2.7.1.67

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  WB analysis using 82938-1-RR (same clone as 82938-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82938-1-RR (PI4KA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82938-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 82938-1-RR (same clone as 82938-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82938-1-RR (PI4KA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82938-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 82938-1-RR (same clone as 82938-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82938-1-RR (PI4KA antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82938-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 82938-1-RR (same clone as 82938-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PI4KA antibody (82938-1-RR, Clone: 230057G2 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 82938-1-PBS in a different storage buffer formulation.

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  FC experiment of MCF-7 using 82938-1-RR (same clone as 82938-1-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug PI4KA Recombinant antibody (82938-1-RR, Clone:230057G2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82938-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82938-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82938-1-RR against Human PI4KA were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

82938-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA PI4KA und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PI4KA fusion protein Ag3062
• Vollständiger Name: phosphatidylinositol 4-kinase, catalytic, alpha
• Berechnetes Molekulargewicht: 2044 aa, 231 kDa
• Beobachtetes Molekulargewicht: 231 kDa
• GenBank-Zugangsnummer: BC018120
• Gene symbol: PI4KA
• Gene ID (NCBI): 5297
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PI4KA, also named as PIK4, PIK4CA, belongs to the PI3/PI4-kinase family and Type III PI4K subfamily. It acts on phosphatidylinositol (PtdIns) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate. PI4KA plays a role in the formation of membrane complexes where HCV replication takes place. (PMID:19605471).