PI4KB Polyklonaler Antikörper

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• KD/KO Validated

PI4KB Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(13)

Kat-Nr. : 13247-1-AP

Synonyme

PtdIns 4-kinase beta, PIK4CB, PI4KIII, PI4K-beta, Phosphatidylinositol 4-kinase beta

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  WB analysis of K-562 using 13247-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13247-1-AP (PI4KB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH-3T3 cells (RNAi) using 13247-1-AP

  WB result of anti- PI4Kb (13247-1-AP) with NIH-3T3 cells (RNAi).

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  WB analysis of Jurkat using 13247-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13247-1-AP (PI4KB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 13247-1-AP

  IP result of anti-PI4KB (IP:13247-1-AP, 4ug; Detection:13247-1-AP 1:300) with K-562 cells lysate 3600ug.

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  IHC staining of human liver using 13247-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13247-1-AP (PI4KB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 13247-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13247-1-AP (PI4KB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of C6 using 13247-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed C6 cells using PI4KB antibody (13247-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, NIH-3T3 Zellen (RNAi)
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: C6-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IF: See 2 publications below

Produktinformation

13247-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA PI4KB und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PI4KB fusion protein Ag3989
• Vollständiger Name: phosphatidylinositol 4-kinase, catalytic, beta
• Berechnetes Molekulargewicht: 801 aa, 90 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC040300
• Gene symbol: PI4KB
• Gene ID (NCBI): 5298
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PI4KB(phosphatidylinositol 4-kinase beta) is also named as PIK4CB, NPIK, PI4K92 and belongs to the PI3/PI4-kinase family. It may regulate Golgi disintegration/reorganization during mitosis, possibly via its phosphorylation and involved in Golgi-to-plasma membrane trafficking. PI4KB is a type III enzyme that is sensitive to wortmannin(PMID:9020160). This protein has 3 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
human,mouse	WB	Cell Res; Intercellular transfer of activated STING triggered by RAB22A-mediated non-canonical autophagy promotes antitumor immunity; Authors - Ying Gao
human	IF	EMBO J; Defining the proximal interaction networks of Arf GTPases reveals a mechanism for the regulation of PLD1 and PI4KB.; Authors - Zhengming Wu
human	WB	PLoS Pathog; Enterovirus 71 protease 2Apro and 3Cpro differentially inhibit the cellular endoplasmic reticulum-associated degradation (ERAD) pathway via distinct mechanisms, and enterovirus 71 hijacks ERAD component p97 to promote its replication.; Authors - Tao Wang
human		Virol Sin; Construction of coxsackievirus B5 viruses with luciferase reporters and their applications in vitro and in vivo; Authors - Shangrui Guo
human	WB,IF	J Virol; N-terminal acetyltransferase 6 facilitates enterovirus 71 replication by regulating PI4KB expression and replication organelle biogenesis; Authors - Hang Yang
mouse	WB	J Neurosci; PI4KIIIβ-Mediated Phosphoinositides Metabolism Regulates Function of the VTA Dopaminergic Neurons and Depression-Like Behavior; Authors - Yuqi Sang