PIAS2 Polyklonaler Antikörper

PIAS2 Polyklonal Antikörper für IF, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 16074-1-AP

Synonyme

ARIP3, DAB2 interacting protein, DIP, E3 SUMO protein ligase PIAS2, miz, MIZ1, PIAS NY protein, PIAS2, PIASX, PIASX ALPHA, PIASX BETA, SIZ2, ZMIZ4

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Galerie der Validierungsdaten

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  WB analysis using 16074-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16074-1-AP (PIAS2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16074-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16074-1-AP (PIAS2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 16074-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 16074-1-AP (PIAS2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 16074-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 16074-1-AP (PIAS2 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, K-562-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IF: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 2 publications below

Produktinformation

16074-1-AP bindet in WB, IF, ELISA PIAS2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PIAS2 fusion protein Ag9032
• Vollständiger Name: protein inhibitor of activated STAT, 2
• Berechnetes Molekulargewicht: 63 kDa, 69 kDa
• Beobachtetes Molekulargewicht: 68 kDa
• GenBank-Zugangsnummer: BC015190
• Gene symbol: PIAS2
• Gene ID (NCBI): 9063
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PIAS2 functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. PIAS2 also plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. PIAS2 has 3 isoforms produced by alternative splicing with the MW of 68 kDa, 63 kDa and 45 kDa.

Publikationen

Species	Application	Title
human	WB,IF	Nat Commun; Identification of proximal SUMO-dependent interactors using SUMO-ID.; Authors - Orhi Barroso-Gomila
human	WB	Biochim Biophys Acta Mol Cell Res; IL-17 promotes melanoma through TRAF2 as a scaffold protein recruiting PIAS2 and ELAVL1 to induce EPHA5; Authors - Junfeng Du
human	WB,IF	J Mol Med (Berl); Hyper-SUMOylation of SMN induced by SENP2 deficiency decreases its stability and leads to spinal muscular atrophy-like pathology.; Authors - Yuhong Zhang