PIAS2 Rekombinanter Antikörper

PIAS2 Rekombinant Antikörper für WB, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241474G8

Kat-Nr. : 84189-4-PBS

Synonyme

241474G8, Androgen receptor-interacting protein 3, ARIP3, DAB2-interacting protein, DIP

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  WB analysis of mouse testis using 84189-4-RR (same clone as 84189-4-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 84189-4-RR (PIAS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84189-4-PBS in a different storage buffer formulation.

• 

  WB analysis using 84189-4-RR (same clone as 84189-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84189-4-RR (PIAS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84189-4-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP01062-1

  Cytometric bead array standard curve of MP01062-1, PIAS2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84189-4-PBS. Detection antibody: 84189-1-PBS. Standard: Ag9032. Range: 0.625-80 ng/mL.

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  Cytometric bead array standard curve of MP01062-2

  Cytometric bead array standard curve of MP01062-2, PIAS2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84189-4-PBS. Detection antibody: 84189-2-PBS. Standard: Ag9032. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 84189-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84189-4-RR against Human PIAS2 were performed. The affinity constant is 0.28 nM.

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Geprüfte Anwendungen

Produktinformation

84189-4-PBS bindet in WB, Cytometric bead array, Indirect ELISA PIAS2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PIAS2 fusion protein Ag9032
• Vollständiger Name: protein inhibitor of activated STAT, 2
• Berechnetes Molekulargewicht: 63 kDa, 69 kDa
• Beobachtetes Molekulargewicht: 63-68 kDa
• GenBank-Zugangsnummer: BC015190
• Gene symbol: PIAS2
• Gene ID (NCBI): 9063
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PIAS2 functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. PIAS2 also plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. PIAS2 has 3 isoforms produced by alternative splicing with the MW of 68 kDa, 63 kDa and 45 kDa.