PIAS2 Rekombinanter Antikörper

PIAS2 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

241474G8

Kat-Nr. : 84189-4-RR

Synonyme

E3 SUMO-protein ligase PIAS2, DIP, DAB2-interacting protein, ARIP3, Androgen receptor-interacting protein 3

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  WB analysis of mouse testis using 84189-4-RR

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 84189-4-RR (PIAS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 84189-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84189-4-RR (PIAS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84189-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84189-4-RR against Human PIAS2 were performed. The affinity constant is 0.28 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, HEK-293T-Zellen, HeLa-Zellen, Maushodengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84189-4-RR bindet in WB, ELISA PIAS2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PIAS2 fusion protein Ag9032
• Vollständiger Name: protein inhibitor of activated STAT, 2
• Berechnetes Molekulargewicht: 63 kDa, 69 kDa
• Beobachtetes Molekulargewicht: 63-68 kDa
• GenBank-Zugangsnummer: BC015190
• Gene symbol: PIAS2
• Gene ID (NCBI): 9063
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PIAS2 functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. PIAS2 also plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. PIAS2 has 3 isoforms produced by alternative splicing with the MW of 68 kDa, 63 kDa and 45 kDa.