PIAS3 Polyklonaler Antikörper

PIAS3 Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 13486-1-AP

Synonyme

E3 SUMO protein ligase PIAS3, PIAS3, ZMIZ5

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 13486-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13486-1-AP (PIAS3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13486-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13486-1-AP (PIAS3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 13486-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13486-1-AP (PIAS3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 13486-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13486-1-AP (PIAS3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 13486-1-AP

  Immunofluorescent analysis of HepG2 cells using 13486-1-AP (PIAS3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 2 publications below

Produktinformation

13486-1-AP bindet in WB, IHC, IF, ELISA PIAS3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PIAS3 fusion protein Ag4304
• Vollständiger Name: protein inhibitor of activated STAT, 3
• Berechnetes Molekulargewicht: 628 aa, 68 kDa
• Beobachtetes Molekulargewicht: 67 kDa
• GenBank-Zugangsnummer: BC030556
• Gene symbol: PIAS3
• Gene ID (NCBI): 10401
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PIAS3(E3 SUMO-protein ligase PIAS3) is a protein of 583 amino acid, has a molecular mass of 68 kDa and belongs to a family of proteins that includes several other members(PMID:11060035). PIAS3 is an inhibitor of activated STAT3 and binds microphthalmia-associated transcription factor, which is a key DNA-binding protein, in rat basophilic leukemia cells and mouse melanocytes(PMID:11709556). The PIAS3 antibody detects PIAS3 (68 kDa) as well as its sumoylated form (85 kDa) in some normal mouse tissues, breast and brain tumor cell lines(PMID:20371673).

Publikationen

Species	Application	Title
human	WB	Mol Med Rep; Role of SUMOylation of STAT1 in tubular epithelial‑mesenchymal transition induced by high glucose; Authors - Cunyang Gu
human	IHC	Int J Mol Sci; Resveratrol Enhances Temozolomide Efficacy in Glioblastoma Cells through Downregulated MGMT and Negative Regulators-Related STAT3 Inactivation; Authors - Moli Wu
human	WB,IHC	Pharmacol Res; PIAS3 promotes ferroptosis by regulating TXNIP via TGF-β signaling pathway in hepatocellular carcinoma; Authors - Wenfang Bao ; KD Validated