PIAS4 Polyklonaler Antikörper

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• KD/KO Validated

PIAS4 Polyklonal Antikörper für WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 14242-1-AP

Synonyme

E3 SUMO protein ligase PIAS4, PIAS gamma, PIAS4, Piasg, PIASY, ZMIZ6

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Galerie der Validierungsdaten

• 

  WB analysis of K-562 using 14242-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14242-1-AP (PIAS4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 14242-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 14242-1-AP (PIAS4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 14242-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 14242-1-AP (PIAS4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 14242-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14242-1-AP (PIAS4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, HL-60-Zellen, Maus-Eierstockgewebe, Maus-Pankreasgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 7 publications below
• IF: See 2 publications below

Produktinformation

14242-1-AP bindet in WB, IF, ELISA PIAS4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PIAS4 fusion protein Ag5498
• Vollständiger Name: protein inhibitor of activated STAT, 4
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 57-70 kDa
• GenBank-Zugangsnummer: BC029874
• Gene symbol: PIAS4
• Gene ID (NCBI): 51588
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PIAS4(protein inhibitor of activated STAT protein 4) is also named as PIASG, PIASy and belongs to the PIAS family. It enhanced the conjugation of SUMO2 to GATA2 through its E3 SUMO ligase activity. TGF-b can up-regulates PLAS4 expression in MCs to down-regulatea-SMA gene transcription by the interaction with E12 and PLAS4 predominantly acts as E3 ligases for E12 in MCs among PIAS family members (PMID:22829926). This protein can be sumoylated and Lys-35 is the main site of sumoylation(PMID:15831457).

Publikationen

Species	Application	Title
human	WB,IF	Nat Commun; Identification of proximal SUMO-dependent interactors using SUMO-ID.; Authors - Orhi Barroso-Gomila
human	WB	Nat Commun; Rad18-dependent SUMOylation of human specialized DNA polymerase eta is required to prevent under-replicated DNA.; Authors - Emmanuelle Despras ; KD Validated
mouse	WB,IF	PLoS Biol; DPPA2/4 and SUMO E3 ligase PIAS4 opposingly regulate zygotic transcriptional program.; Authors - Yao-Long Yan ; KD Validated
mouse	WB	PLoS One; Protein inhibitor of activated STAT, PIASy regulates α-smooth muscle actin expression by interacting with E12 in mesangial cells.; Authors - Torikoshi Kazuo K ; KD Validated
human	WB	Front Nutr; Hengshun Aromatic Vinegar Ameliorates Vascular Endothelial Injury via Regulating PKCζ-Mediated Oxidative Stress and Apoptosis.; Authors - Xin Li
human	WB	Nat Commun; Suppression of ACE2 SUMOylation protects against SARS-CoV-2 infection through TOLLIP-mediated selective autophagy; Authors - Shouheng Jin ; KD Validated