PIBF1 Polyklonaler Antikörper

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• KD/KO Validated

PIBF1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14413-1-PBS

Synonyme

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  WB analysis of HeLa using 14413-1-AP (same clone as 14413-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 14413-1-AP (PIBF1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14413-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 14413-1-AP (same clone as 14413-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14413-1-AP (PIBF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14413-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 14413-1-AP (same clone as 14413-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14413-1-AP (PIBF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14413-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 14413-1-AP (same clone as 14413-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 14413-1-AP (PIBF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14413-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HEK-293 using 14413-1-AP (same clone as 14413-1-PBS)

  IP result of anti-PIBF1 (IP:14413-1-AP, 4ug; Detection:14413-1-AP 1:1000) with HEK-293 cells lysate 1400 ug. This data was developed using the same antibody clone with 14413-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 14413-1-AP (same clone as 14413-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 14413-1-AP (PIBF1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14413-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 14413-1-AP (same clone as 14413-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using PIBF1 antibody (14413-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Gamma Tubulin antibody (66320-1-Ig, Clone: 3F9H8, red). This data was developed using the same antibody clone with 14413-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

14413-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA PIBF1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PIBF1 fusion protein Ag5755
• Vollständiger Name: progesterone immunomodulatory binding factor 1
• Berechnetes Molekulargewicht: 90 kDa
• Beobachtetes Molekulargewicht: 90 kDa
• GenBank-Zugangsnummer: BC051911
• Gene symbol: PIBF1
• Gene ID (NCBI): 10464
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PIBF1 is induced by the steroid hormone progesterone and plays a role in the maintenance of pregnancy. PIBF1 regulates multiple facets of the immune system to promote normal pregnancy including cytokine synthesis, natural killer (NK) cell activity, and arachidonic acid metabolism. Low serum levels of this protein have been associated with spontaneous pre-term labor in humans. PIBF1 may promote the proliferation, migration and invasion of glioma.