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PICALM Polyklonaler Antikörper

PICALM Polyklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 28554-1-PBS

Synonyme

CALM, CLTH, LAP, Phosphatidylinositol-binding clathrin assembly protein

Filter:
  • Western Blot (WB) analysis of various lysates using PICALM Polyclonal antibody (28554-1-AP)

    WB analysis using 28554-1-AP (same clone as 28554-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28554-1-AP (PICALM antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28554-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PICALM Polyclonal antibody (28554-1-AP)

    IHC staining of mouse brain using 28554-1-AP (same clone as 28554-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28554-1-AP (PICALM antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28554-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using PICALM Polyclonal antibody (28554-1-AP)

    IF Staining of mouse brain using 28554-1-AP (same clone as 28554-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using PICALM antibody (28554-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28554-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

28554-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA PICALM und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen PICALM fusion protein Ag29505
Vollständiger Name phosphatidylinositol binding clathrin assembly protein
Berechnetes Molekulargewicht 71 kDa
Beobachtetes Molekulargewicht 60-70 kDa
GenBank-ZugangsnummerBC064357
Gene symbol PICALM
Gene ID (NCBI) 8301
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PICALM (Phosphatidyl Inositol Clathrin Assembly Lymphoid Myeloid protein) is a ubiquitously expressed protein that plays a role in clathrin-mediated endocytosis. PICALM also affects the internalization and trafficking of SNAREs and modulates macroautophagy. PICALM is a highly validated genetic risk factor for Alzheimer's disease (AD). The long isoform of human PICALM has 22 exons (encoding PICALML), while the short isoform lacks exon 14 (encoding PICALMS). (PMID: 33397371, PMID: 26075887, PMID: 26005850)