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PICALM Monoklonaler Antikörper

PICALM Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1F6G7

Kat-Nr. : 67564-1-PBS

Synonyme

1F6G7, CALM, CLTH, LAP, Phosphatidylinositol-binding clathrin assembly protein

Filter:
  • Western Blot (WB) analysis of various lysates using PICALM Monoclonal antibody (67564-1-Ig)

    WB analysis using 67564-1-Ig (same clone as 67564-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67564-1-Ig (PICALM antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67564-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PICALM Monoclonal antibody (67564-1-Ig)

    IHC staining of human lung cancer using 67564-1-Ig (same clone as 67564-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67564-1-Ig (PICALM antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67564-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PICALM Monoclonal antibody (67564-1-Ig)

    IF Staining of HeLa using 67564-1-Ig (same clone as 67564-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PICALM antibody (67564-1-Ig, Clone: 1F6G7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67564-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PICALM Monoclonal antibody (67564-1-Ig)

    IF Staining of HepG2 using 67564-1-Ig (same clone as 67564-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PICALM antibody (67564-1-Ig, Clone: 1F6G7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67564-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

67564-1-PBS bindet in WB, IHC, IF/ICC, ELISA PICALM und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen PICALM fusion protein Ag29512
Vollständiger Name phosphatidylinositol binding clathrin assembly protein
Berechnetes Molekulargewicht 71 kDa
Beobachtetes Molekulargewicht 60-70 kDa
GenBank-ZugangsnummerBC064357
Gene symbol PICALM
Gene ID (NCBI) 8301
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PICALM (Phosphatidyl Inositol Clathrin Assembly Lymphoid Myeloid protein) is a ubiquitously expressed protein that plays a role in clathrin-mediated endocytosis. PICALM also affects the internalization and trafficking of SNAREs and modulates macroautophagy. PICALM is a highly validated genetic risk factor for Alzheimer's disease (AD). The long isoform of human PICALM has 22 exons (encoding PICALML), while the short isoform lacks exon 14 (encoding PICALMS). (PMID: 33397371, PMID: 26075887, PMID: 26005850)