PICK1 Polyklonaler Antikörper

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• KD/KO Validated

PICK1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10983-2-PBS

Synonyme

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  WB analysis using 10983-2-AP (same clone as 10983-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10983-2-AP (PICK1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10983-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 10983-2-AP (same clone as 10983-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10983-2-AP (PICK1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10983-2-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 10983-2-AP (same clone as 10983-2-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 10983-2-AP (PICK1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10983-2-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 10983-2-AP (same clone as 10983-2-PBS)

  IP result of anti-PICK1 (IP:10983-2-AP, 4ug; Detection:10983-2-AP 1:2500) with mouse brain tissue lysate 1280 ug. This data was developed using the same antibody clone with 10983-2-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 10983-2-AP (same clone as 10983-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10983-2-AP (PICK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10983-2-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 10983-2-AP (same clone as 10983-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10983-2-AP (PICK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10983-2-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 10983-2-AP (same clone as 10983-2-PBS)

  Immunofluorescent analysis of HEK-293 cells using 10983-2-AP (PICK1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10983-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10983-2-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA PICK1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PICK1 fusion protein Ag1443
• Vollständiger Name: protein interacting with PRKCA 1
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC017561
• Gene symbol: PICK1
• Gene ID (NCBI): 9463
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein interacting with C kinase 1 (PICK1) was first cloned as a PKC-binding partner through yeast two hybrid system. PICK1 acts as a critical regulator of membrane receptors' subcellular trafficking to modulate neural processes such as learning and memory, and is widely expressed in brain, testis, heart, lung, liver, kidney and muscle. It probably binds to and organize the subcellular localization of a variety of membrane proteins containing some PDZ recognition sequence, for instance, PICK1 is a critical mediator of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) trafficking in neural synapses. PICK1 expression on D-serine release and glutamate transport in astrocytes suggests a potential implication of PICK1 in the progression of amyotrophic lateral sclerosis (ALS). PICK1 may also participate in breast cancer development through inhibition of TGF-β signaling.