PICK1 Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

PICK1 Rekombinant Antikörper für WB, IHC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

230185B8

Kat-Nr. : 82919-1-RR

Synonyme

230185B8, PICK, PRKCA binding protein, PRKCA-binding protein, PRKCABP

---

• 

  WB analysis using 82919-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82919-1-RR (PICK1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 82919-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82919-1-RR (PICK1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 82919-1-RR

  WB result of PICK1 antibody (82919-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PICK1 transfected HEK-293 cells.

• 

  IHC staining of human stomach cancer using 82919-1-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 82919-1-RR (PICK1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 82919-1-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 82919-1-RR (PICK1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 82919-1-RR

  Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 82919-1-RR (PICK1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HEK-293T using 82919-1-RR

  1x10^6 HEK-293T cells were intracellularly stained with 0.25 ug Anti-Human PICK1 (82919-1-RR, Clone:230185B8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166 (red), or 0.25 ug rabbit IgG isotype control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  Affinity and Kinetic Characterization of 82919-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82919-1-RR against Human PICK1 were performed. The affinity constant is 0.792 nM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, HEK-293-Zellen, HeLa-Zellen, MCF-7-Zellen, Maushirngewebe, Hausschwein-Hirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC (Intra): HEK-293T-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:400-1:1600
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82919-1-RR bindet in WB, IHC, FC (Intra), ELISA PICK1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PICK1 fusion protein Ag1443
• Vollständiger Name: protein interacting with PRKCA 1
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC017561
• Gene symbol: PICK1
• Gene ID (NCBI): 9463
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein interacting with C kinase 1 (PICK1) was first cloned as a PKC-binding partner through yeast two hybrid system. PICK1 acts as a critical regulator of membrane receptors' subcellular trafficking to modulate neural processes such as learning and memory, and is widely expressed in brain, testis, heart, lung, liver, kidney and muscle. It probably binds to and organize the subcellular localization of a variety of membrane proteins containing some PDZ recognition sequence, for instance, PICK1 is a critical mediator of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) trafficking in neural synapses. PICK1 expression on D-serine release and glutamate transport in astrocytes suggests a potential implication of PICK1 in the progression of amyotrophic lateral sclerosis (ALS). PICK1 may also participate in breast cancer development through inhibition of TGF-β signaling.