PIGA Polyklonaler Antikörper

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• KD/KO Validated

PIGA Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 13679-1-AP

Synonyme

GlcNAc PI synthesis protein, GPI3, PIG A, PIGA

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  WB analysis of HeLa using 13679-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13679-1-AP (PIGA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 13679-1-AP

  WB result of PIGA antibody (13679-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PIGA transfected HEK-293 cells.

• 

  WB analysis of HeLa using 13679-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13679-1-AP (PIGA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse lung using 13679-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 13679-1-AP (PIGA antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 13679-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 13679-1-AP (PIGA antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 13679-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 13679-1-AP (PIGA antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 13679-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 13679-1-AP (PIGA antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen
• Erfolgreiche Detektion in IHC: Mauslungengewebe, Rattenlebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

13679-1-AP bindet in WB, IHC, IF, ELISA PIGA und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PIGA fusion protein Ag4530
• Vollständiger Name: phosphatidylinositol glycan anchor biosynthesis, class A
• Berechnetes Molekulargewicht: 484 aa, 54 kDa
• Beobachtetes Molekulargewicht: 38 kDa
• GenBank-Zugangsnummer: BC038236
• Gene symbol: PIGA
• Gene ID (NCBI): 5277
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	IF,WB	Hum Mol Genet; CNS glycosylphosphatidylinositol deficiency results in delayed white matter development, ataxia and premature death in a novel mouse model.; Authors - Marshall Lukacs ; KO Validated
Mouse	IHC	Front Genet; Machine learning potential predictor of idiopathic pulmonary fibrosis; Authors - Chenchun Ding