PIGK Rekombinanter Antikörper

PIGK Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241989D4

Kat-Nr. : 84568-3-PBS

Synonyme

hGPI8, GPI-anchor transamidase, GPI8, GPI transamidase, EC:3.-.-.-

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  WB analysis using 84568-3-RR (same clone as 84568-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84568-3-RR (PIGK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84568-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84568-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84568-3-RR against Human PIGK were performed. The affinity constant is 0.501 nM.

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Geprüfte Anwendungen

Produktinformation

84568-3-PBS bindet in WB, Indirect ELISA PIGK und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PIGK fusion protein Ag3497
• Vollständiger Name: phosphatidylinositol glycan anchor biosynthesis, class K
• Berechnetes Molekulargewicht: 45 kDa
• Beobachtetes Molekulargewicht: 40-45 kDa
• GenBank-Zugangsnummer: BC026186
• Gene symbol: PIGK
• Gene ID (NCBI): 10026
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Phosphatidylinositol glycan, class K (PIGK), is a crucial member of the glycosyl-phosphatidylinositol transamidase (GPIT) protein complex that attaches a diverse group of macromolecules to the plasma membrane of eukaryotes. The human PIGK gene is involved in the key step of transferring GPI-anchor to the respective protein molecules in the plasma membrane (PMID: 22824918). Endogenous PIGK has 2 isoforms, 45 kDa and 36 kDa (PMID: 34193731).