PIGO Polyklonaler Antikörper

PIGO Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 16369-1-AP

Synonyme

UNQ632/PRO1249, RP11 182N22.4, PIG-O, PIG O, Phosphatidylinositol-glycan biosynthesis class O protein

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  WB analysis of HeLa using 16369-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16369-1-AP (PIGO antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 16369-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 16369-1-AP (PIGO antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 16369-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 16369-1-AP (PIGO antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 16369-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 16369-1-AP (PIGO antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 16369-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16369-1-AP (PIGO antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 16369-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16369-1-AP (PIGO antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 16369-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16369-1-AP (PIGO antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 16369-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16369-1-AP (PIGO antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 16369-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16369-1-AP (PIGO antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 16369-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16369-1-AP (PIGO antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 16369-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16369-1-AP (PIGO antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 16369-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16369-1-AP (PIGO antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 16369-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16369-1-AP (PIGO antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 16369-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16369-1-AP (PIGO antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of MCF-7 using 16369-1-AP

  Immunofluorescent analysis of MCF-7 cells, using PIGO antibody 16369-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A431-Zellen, COLO 320-Zellen, Mausmilzgewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Nierengewebe, humanes Hautgewebe, humanes Milzgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

16369-1-AP bindet in WB, IHC, IF/ICC, ELISA PIGO und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PIGO fusion protein Ag9232
• Vollständiger Name: phosphatidylinositol glycan anchor biosynthesis, class O
• Berechnetes Molekulargewicht: 1089aa,119 kDa; 454aa,50 kDa
• Beobachtetes Molekulargewicht: 74 kDa
• GenBank-Zugangsnummer: BC013987
• Gene symbol: PIGO
• Gene ID (NCBI): 84720
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
Mouse	WB	Oxid Med Cell Longev; Nrf2 Activation Attenuates Chronic Constriction Injury-Induced Neuropathic Pain via Induction of PGC-1α-Mediated Mitochondrial Biogenesis in the Spinal Cord.; Authors - Jia Sun
Mouse		CNS Neurosci Ther; Attenuation of neuronal ferroptosis in intracerebral hemorrhage by inhibiting HDAC1/2: Microglial heterogenization via the Nrf2/HO1 pathway; Authors - Zhiwen Jiang
		Br J Pharmacol; Inhibition of Brd4 alleviates osteoarthritis pain via suppression of neuroinflammation and activation of Nrf2-mediated antioxidant signalling; Authors - Jia Sun