PIGS Polyklonaler Antikörper

PIGS Polyklonal Antikörper für IF, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF,ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 18334-1-AP

Synonyme

PIGS

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Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 18334-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18334-1-AP (PIGS Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 18334-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18334-1-AP (PIGS Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  WB analysis of HepG2 using 18334-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18334-1-AP (PIGS Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 18334-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 18334-1-AP (PIGS Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 18334-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18334-1-AP (PIGS Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 18334-1-AP

  Immunofluorescent analysis of HepG2 cells using 18334-1-AP (PIGS antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, HepG2-Zellen, Mauslebergewebe, PC-3-Zellen
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

18334-1-AP bindet in WB, IF,ELISA PIGS und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PIGS fusion protein Ag12950
• Vollständiger Name: phosphatidylinositol glycan anchor biosynthesis, class S
• Berechnetes Molekulargewicht: 62 kDa
• Beobachtetes Molekulargewicht: 65-70 kDa
• GenBank-Zugangsnummer: BC001319
• Gene symbol: PIGS
• Gene ID (NCBI): 94005
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PIGS, also named as GPI transamidase component PIG-S or PRO4316, is a 555 amino acid protein, which belongs to the PIGS family. PIGS localizes in the endoplasmic reticulum membrane. PIGS is a component of the GPI transamidase complex. It is essential for transfer of GPI to proteins, particularly for formation of carbonyl intermediates.

Publikationen

Species	Application	Title
human	WB	J Biol Chem; Identification of mammalian glycoproteins with type-I LacdiNAc structures synthesized by the glycosyltransferase B3GALNT2.; Authors - Takahiro Nakane