PIGS Polyklonaler Antikörper

PIGS Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18334-1-PBS

Synonyme

UNQ1873/PRO4316, Phosphatidylinositol-glycan biosynthesis class S protein, GPI transamidase component PIG-S

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  WB analysis of HeLa using 18334-1-AP (same clone as 18334-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 18334-1-AP (PIGS Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18334-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 18334-1-AP (same clone as 18334-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18334-1-AP (PIGS Antibody) at dilution of 1:300 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 18334-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 18334-1-AP (same clone as 18334-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18334-1-AP (PIGS Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18334-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 18334-1-AP (same clone as 18334-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 18334-1-AP (PIGS Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18334-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 18334-1-AP (same clone as 18334-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18334-1-AP (PIGS Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18334-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 18334-1-AP (same clone as 18334-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PIGS antibody (18334-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 18334-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

18334-1-PBS bindet in WB, IF/ICC, Indirect ELISA PIGS und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PIGS fusion protein Ag12950
• Vollständiger Name: phosphatidylinositol glycan anchor biosynthesis, class S
• Berechnetes Molekulargewicht: 62 kDa
• Beobachtetes Molekulargewicht: 65-70 kDa
• GenBank-Zugangsnummer: BC001319
• Gene symbol: PIGS
• Gene ID (NCBI): 94005
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PIGS, also named as GPI transamidase component PIG-S or PRO4316, is a 555 amino acid protein, which belongs to the PIGS family. PIGS localizes in the endoplasmic reticulum membrane. PIGS is a component of the GPI transamidase complex. It is essential for transfer of GPI to proteins, particularly for formation of carbonyl intermediates.