PIGT Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PIGT Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 16906-1-AP

Synonyme

CGI-06, GPI transamidase component PIG-T, NDAP, Phosphatidylinositol-glycan biosynthesis class T protein, PSEC0163

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  WB analysis of human brain using 16906-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16906-1-AP (PIGT antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 16906-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 16906-1-AP (PIGT antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 16906-1-AP

  IP result of anti-PIGT (IP:16906-1-AP, 4ug; Detection:16906-1-AP 1:300) with mouse liver tissue lysate 6000 ug.

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  IHC staining of human brain using 16906-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16906-1-AP (PIGT antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 16906-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16906-1-AP (PIGT antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of mouse brain using 16906-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16906-1-AP (PIGT antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 16906-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PIGT antibody (16906-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, A549-Zellen
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Hirngewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 1 publications below

Produktinformation

16906-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA PIGT und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PIGT fusion protein Ag10521
• Vollständiger Name: phosphatidylinositol glycan anchor biosynthesis, class T
• Berechnetes Molekulargewicht: 578 aa, 66 kDa
• Beobachtetes Molekulargewicht: 66 kDa
• GenBank-Zugangsnummer: BC015022
• Gene symbol: PIGT
• Gene ID (NCBI): 51604
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PIGT is a subunit of the glycosylphosphatidylinositol transamidase complex that catalyzes the attachment of proteins to GPI-anchors. GPI-anchors are glycolipids found on membrane of diverse cells that help proteins anchoring to the cell surface. Mutations of PIGT have been linked to developmental disorders including multiple congenital anomalies-hypotonia-seizures syndrome-3. Multiple isoforms of PIGT exist due to the alternative splicing, with the predicted MWs ranging from 42 kDa to 66 kDa.

Publikationen

Species	Application	Title
human	WB	J Transl Med; PIGT promotes cell growth, glycolysis, and metastasis in bladder cancer by modulating GLUT1 glycosylation and membrane trafficking; Authors - Mingyue Tan ; KD Validated
mouse	WB,IF	CNS Neurosci Ther; Integrated transcriptomic and proteomic profiling reveals the key molecular signatures of brain endothelial reperfusion injury; Authors - Yabin Ji
mouse,human	WB	Nat Commun; Proteomic screens of SEL1L-HRD1 ER-associated degradation substrates reveal its role in glycosylphosphatidylinositol-anchored protein biogenesis; Authors - Xiaoqiong Wei
mouse	WB	BMC Med Genomics; Proteomic analysis illustrates the potential involvement of dysregulated ribosome-related pathways and disrupted metabolism during retinoic acid-induced cleft palate development; Authors - Wancong Zhang