PIH1D1 Polyklonaler Antikörper

PIH1D1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19427-1-PBS

Synonyme

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  WB analysis using 19427-1-AP (same clone as 19427-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 19427-1-AP (PIH1D1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19427-1-PBS in a different storage buffer formulation.

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  WB analysis of human testis using 19427-1-AP (same clone as 19427-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 19427-1-AP (PIH1D1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19427-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse eye using 19427-1-AP (same clone as 19427-1-PBS)

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 19427-1-AP (PIH1D1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19427-1-PBS in a different storage buffer formulation.

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  WB analysis of human uterus using 19427-1-AP (same clone as 19427-1-PBS)

  human uterus tissue were subjected to SDS PAGE followed by western blot with 19427-1-AP (PIH1D1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19427-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 19427-1-AP (same clone as 19427-1-PBS)

  IP result of anti-PIH1D1 (IP:19427-1-AP, 4ug; Detection:19427-1-AP 1:1000) with HeLa cells lysate 1200ug. This data was developed using the same antibody clone with 19427-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung using 19427-1-AP (same clone as 19427-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung using 19427-1-AP (PIH1D1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 19427-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung using 19427-1-AP (same clone as 19427-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung using 19427-1-AP (PIH1D1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 19427-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary using 19427-1-AP (same clone as 19427-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary using 19427-1-AP (PIH1D1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 19427-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary using 19427-1-AP (same clone as 19427-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary using 19427-1-AP (PIH1D1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 19427-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 19427-1-AP (same clone as 19427-1-PBS)

  Immunofluorescent analysis of Hela cells, using PIH1D1 antibody 19427-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 19427-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

19427-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA PIH1D1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PIH1D1 fusion protein Ag13759
• Vollständiger Name: PIH1 domain containing 1
• Berechnetes Molekulargewicht: 290 aa, 32 kDa
• Beobachtetes Molekulargewicht: 39 kDa
• GenBank-Zugangsnummer: BC001108
• Gene symbol: PIH1D1
• Gene ID (NCBI): 55011
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PIH1D1 (PIH1 domain-containing protein 1; also known as PIH1 or NOP17), is a ortholog of the yeast nucleolar protein NOP17. PIH1D1 is a conserved subunit of the R2TP complex participating in the assembly of snoRNP and RNA polymerase II complexes. PIH1D1 may also function as a novel modulator of apoptosis pathway.